FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_NEGATIVE_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_NEGATIVE_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7813
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT3123.9933444259567388No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT1451.8558812236016895RNA PCR Primer, Index 27 (95% over 23bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG1391.7790861384871368RNA PCR Primer, Index 27 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT1121.4335082554716498RNA PCR Primer, Index 27 (96% over 25bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT650.8319467554076538RNA PCR Primer, Index 27 (96% over 28bp)
GTAGTAACAAGAGCGGGGGGAGCAGAAGCACGCACTTGGGGGGAGCAGAA460.5887623192115704No Hit
GTAGTAACAAGAGCCGGGTTATTAGTAGAAACAACGCACCGGGTTATTAG420.5375655958018687No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA330.4223729681300397RNA PCR Primer, Index 27 (96% over 30bp)
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT250.3199795213106361RNA PCR Primer, Index 27 (95% over 22bp)
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTG210.26878279790093434RNA PCR Primer, Index 27 (100% over 24bp)
GTAGTAACAAGAGCTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATT200.2559836170485089No Hit
CTTCTGCTCCCCCCGCTCCGCTTCTGCTCCTGTCTCTTATACACATCTCC170.21758607449123257No Hit
GAAGCGGAGCGGGGGGAGCAGAAGCACGCTCGTGTTTCTACCTGTCTCTT160.20478689363880712No Hit
GAAGCGGAGCGGGGGGAGCAGAAGCACGCACGTGTTTCTACCTGTCTCTT160.20478689363880712No Hit
GAGCAGAAGCGGAGCGGGGGGAGCAGAAGCTGTCTCTTATACACATCTCC160.20478689363880712No Hit
GAAGCGGAGCGGGGGGAGCAGAAGCAGGCACGTGTTTCTACCTGTCTCTT140.17918853193395623No Hit
AGTAACAAGAGCTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTAG140.17918853193395623No Hit
CTTCTGCTCCCCCCGCTCCGCTTCTGCTCTGTCTCTTATACACATCTCCG140.17918853193395623No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTAACTCGTATGCCGT140.17918853193395623No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC130.16638935108153077No Hit
AAACACGAGCGTGCTTCTGCTCCCCCCGCTCCGCTTCCTGTCTCTTATAC130.16638935108153077No Hit
ACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGC130.16638935108153077TruSeq Adapter, Index 27 (96% over 25bp)
GTAGAAACACGTGCGTGCTTCTGCTCCCCCCGCTCCGCTTCCTGTCTCTT120.15359017022910534No Hit
ATTAGTAGTAACAAGAGCTCCGGGTTATTAGTAGTAACAAGAGCCCGGGT120.15359017022910534No Hit
ATTAGTAGTAACAAGAGCCGGGTTATTAGTAGAAACAACGCACCGGGTTA120.15359017022910534No Hit
GTAGAAACACGAGCGTGCTTCTGCTCCCCCCGCTCCGCTTCCTGTCTCTT120.15359017022910534No Hit
GTAGAAACACGAGCCTGCTTCTGCTCCCCCCGCTCCGCTTCCTGTCTCTT110.1407909893766799No Hit
GTAGAAACACGAGCTCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATT110.1407909893766799No Hit
AACAAGAGCCGGGTTATTAGTAGAAACAACGCACCGGGTTATTAGTAGAA110.1407909893766799No Hit
ACTAATAACCCGGTGCGTTGTTTCTACTAATAACCCGGCTCTTGTTACTA100.12799180852425446No Hit
GTAGAAACACGTGCCTGCTTCTGCTCCCCCCGCTCCGCTTCCTGTCTCTT100.12799180852425446No Hit
GAGCAGAAGCACGCACGTGTTTCTACTAATAACCCGGGCTCTTGTTACTA100.12799180852425446No Hit
AGTAACAAGAGCCGGGTTATTAGTAGAAACAACGCACCGGGTTATTAGTA90.115192627671829No Hit
GAGCAGAAGCGGAGCGGGGGGAGCAGAAGCACGCTCGTGTTTCTGTCTCT90.115192627671829No Hit
TATTAGTAGTAACAAGAGCCGGGTTATTAGTAGAAACAACGCACCGGGTT90.115192627671829No Hit
GTAGAAACACGAGCCTGCTTCTGCTCCCCCCAAGTGCGTGCTTCTGCTCC90.115192627671829No Hit
GAGCAGAAGCGGAGCTCGTGTTTCTACTAATAACCCGGGCTCTTGTTACT90.115192627671829No Hit
CTTGTTACTACTAATAACCCGGAGTAACAAGAGCCCGGGTTATTAGTAGA90.115192627671829No Hit
GAAGCGGAGCGGGGGGAGCAGAAGCAGGCTCGTGTTTCTACCTGTCTCTT90.115192627671829No Hit
CTAATAACCCGGTGCGTTGTTTCTACTAATAACCCGGCTCTTGTTACTAC90.115192627671829No Hit
GTAGAAACACGAGCTTCTGCTCCCCCCGCTCCGCTTCCTGTCTCTTATAC80.10239344681940356No Hit
CTAATAACCCGGGCTCTTGTTACTACTAATAACCCGGAGCTCTTGTTACT80.10239344681940356No Hit
GTAGAAACACGAGCGTGCTTCTGCTCCCCCCGCTCCGCTTCTGCTCTGTC80.10239344681940356No Hit
TGTTTCTACTAATAACCCGGTGCGTTGTTTCTACTAATAACCCGGCTCTT80.10239344681940356No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTTGT100.0069574947145.01
CACTGGG100.0069574947145.07
ACGAGCC405.564416E-790.6259
TAGTAAC502.0986772E-672.52
GTAGTAA502.0986772E-672.51
AGAAGCG702.0463813E-762.1428574
AGTAACA659.9588E-655.769233
TAACAAG701.544718E-551.7857135
GTAACAA701.544718E-551.7857134
GAAGCGG901.161432E-648.3333325
GAAACAC1750.045.571434
CAAGAGC803.4030618E-545.31258
CGGAGCG803.4030618E-545.31259
AAACACG1801.8189894E-1244.3055575
AACACGA1151.1870725E-744.1304366
AAGCGGA854.8683098E-542.647066
GTAGAAA2050.042.4390261
TAGAAAC2100.041.428572
ACACGAG1252.2862696E-740.6000027
CACGAGC1252.2862696E-740.6000028
Produced by FastQC (version 0.11.5)