FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_DEMUX_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_DEMUX_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5864
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT3335.6787175989085945No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT1682.8649386084583903TruSeq Adapter, Index 3 (95% over 21bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG1682.8649386084583903TruSeq Adapter, Index 3 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT1452.4727148703956345RNA PCR Primer, Index 3 (95% over 23bp)
GACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCACCTAT981.6712141882673943No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT751.2789904502046385TruSeq Adapter, Index 3 (96% over 26bp)
GTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACA591.0061391541609823No Hit
GTAGAAACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCC591.0061391541609823No Hit
ACCATGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGG530.9038199181446112No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA460.7844474761255117RNA PCR Primer, Index 3 (96% over 28bp)
GTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTCT430.7332878581173261No Hit
ATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTCTGAATC390.665075034106412No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG360.6139154160982265TruSeq Adapter, Index 3 (95% over 24bp)
AAGCAGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCA330.562755798090041No Hit
CCTGTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGG330.562755798090041No Hit
GTAGTAACAAGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCC310.5286493860845839No Hit
GTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGGATGTCAAGACC290.4945429740791269No Hit
GGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTG280.47748976807639837No Hit
GCAGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGG280.47748976807639837No Hit
GTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCCTTCGGGACA280.47748976807639837No Hit
CCTAGAGATAGGTGCGGCCTTCGGGACAGAGGAGACAGGTGGTGCATGGT280.47748976807639837No Hit
CCTCTGAATCACCTAGAGATAGGTGCGGCCTTCGGGACAGAGGAGACAGG250.42633015006821284No Hit
GTAGAAACACGTGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCC250.42633015006821284No Hit
GAACCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCG240.4092769440654843No Hit
ATTAGTAGAAACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCTG240.4092769440654843No Hit
AGCAGAAGCAGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCT240.4092769440654843No Hit
GGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCCTTCGGGAC240.4092769440654843No Hit
TCTCTAGGTGATTCAGAGGGATGTCAAGACCTGGTAAGGTTCTTCGCGTT230.39222373806275584No Hit
CCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCC210.3581173260572988No Hit
ATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATC210.3581173260572988No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC200.34106412005457026No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT200.34106412005457026No Hit
CCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGGATGTCA190.32401091405184174No Hit
TACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCCTTC190.32401091405184174No Hit
GCAGAGGATTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGG180.30695770804911326No Hit
ATTAGTAGTAACAAGAGCTGACGACAACCATGCACCACCTGTCTCCTCTG170.2899045020463847No Hit
GAAGCAGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACC170.2899045020463847No Hit
GCGGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGG160.2728512960436562No Hit
CGAAGAACCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGG150.25579809004092774No Hit
ATCTCTAGGTGATTCAGAGGGATGTCAAGACCTGGTAAGGTTCTTCGCGT140.23874488403819918No Hit
AACAAGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGG130.22169167803547069No Hit
GAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCT130.22169167803547069No Hit
AGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGAC130.22169167803547069No Hit
GAGCAGAAGCAGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACC120.20463847203274216No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAACCTCGTATGCCGT120.20463847203274216No Hit
GAAGCGGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACC120.20463847203274216No Hit
TGTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGGA120.20463847203274216No Hit
AACCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGG120.20463847203274216No Hit
GGATTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTG110.18758526603001366No Hit
AAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTCTGAATCACCTA110.18758526603001366No Hit
GCGGTGGAGCATGTGGTTTAATTCGAAGCTACGCGAAGAACCTTACCAGG110.18758526603001366No Hit
AGTAACAAGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCCGA110.18758526603001366No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGC100.17053206002728513Illumina PCR Primer Index 12 (95% over 24bp)
AGAGATAGGTGCGGCCTTCGGGACAGAGGAGACAGGTGGTGCATGGTTGT100.17053206002728513No Hit
CGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCACCTATCT100.17053206002728513No Hit
GTAACAAGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAA100.17053206002728513No Hit
AATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTCTGAAT100.17053206002728513No Hit
CTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCCTTCGGGACAGA100.17053206002728513No Hit
TTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGCCTT90.15347885402455663No Hit
GTAGAAACACGAGCTGACGACAACCATGCACCACCTGTATCCATGTCCCC90.15347885402455663No Hit
GACGACAACCATGCACCACCTGTATCCATGTCCCCGAAGGGAACGTCTAA90.15347885402455663No Hit
GTAGTAACAAGAGCCCGGGTTATTAGTAGTAACAAGAGCCCGGGTTATTA90.15347885402455663No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGTAGAGG90.15347885402455663No Hit
GGCCGCACCTATCTCTAGGTGATTCAGAGGGATGTCAAGACCTGGTAAGG90.15347885402455663No Hit
TATTAGTAGTAACAAGAGCTGACGACAACCATGCACCACCTGTCTCCTCT90.15347885402455663No Hit
GCGAAGAACCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAG80.1364256480218281No Hit
ATTCAGAGGGATGTCAAGACCTGGTAAGGTTCTTCGCGTTGCTTCGAATT80.1364256480218281No Hit
CTGTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGG80.1364256480218281No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGT80.1364256480218281No Hit
GAGATAGGTGCGGCCTTCGGGACAGAGGAGACAGGTGGTGCATGGTTGTC80.1364256480218281No Hit
AAGCGGAGGAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCA80.1364256480218281No Hit
TTAGTAGAAACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGT80.1364256480218281No Hit
ATTAGTAGAAACACGTGCTGACGACAACCATGCACCACCTGTCTCCTCTG80.1364256480218281No Hit
TTAGTAGAAACACGTGCTGACGACAACCATGCACCACCTGTCTCCTCTGT70.11937244201909959No Hit
GGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGACATCCCTC70.11937244201909959No Hit
CTCTAGGTGATTCAGAGGGATGTCAAGACCTGGTAAGGTTCTTCGCGTTG70.11937244201909959No Hit
CCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGGATGTCAAGACCTG70.11937244201909959No Hit
ACCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGATAGGTGCGGC70.11937244201909959No Hit
GAAGCAGAGGATTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACC70.11937244201909959No Hit
AGATAGGTGCGGCCTTCGGGACAGAGGAGACAGGTGGTGCATGGTTGTCG60.10231923601637108No Hit
AACACGTGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGG60.10231923601637108No Hit
GGTGCGGCCTTCGGGACAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGC60.10231923601637108No Hit
GAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCA60.10231923601637108No Hit
CACCTAGAGATAGGTGCGGCCTTCGGGACAGAGGAGACAGGTGGTGCATG60.10231923601637108No Hit
ACGCGAAGAACCTTACCAGGTCTTGACATCCCTCTGAATCACCTAGAGAT60.10231923601637108No Hit
GCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAGGCCGCACC60.10231923601637108No Hit
ATTCGAAGCTACGCGAAGAACCTTACCAGGTCTTGACATACTATGCAAAT60.10231923601637108No Hit
AAACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCCCGAAG60.10231923601637108No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAACCTCGTATGCCGTCTT60.10231923601637108No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAAACTCGTATGCCGTCTTCTGCT60.10231923601637108Illumina PCR Primer Index 8 (95% over 24bp)
GAGTATGTGGTTTAATTCGAAGCAACGCGAAGAACCTTACCAGGTCTTGA60.10231923601637108No Hit
TTGACATACTATGCAAATCTAAGAGATTAGACGTTCCCTTCGGGGACATG60.10231923601637108No Hit
ACCATGCACCACCTGTATCCATGTCCCCGAAGGGAACGTCTAATCTCTTA60.10231923601637108No Hit
GAGCAGAAGCAGAGGATTATGTGGTTTAATTCGAAGCAACGCGAAGAACC60.10231923601637108No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATATCGTATGCCGT60.10231923601637108No Hit
GTCTCCTCTGTCCCGAAGGCCGCACCTATCTCTAGGTGATTCAGAGGGAT60.10231923601637108No Hit
CTTCGGGACAGAGGAGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTTT60.10231923601637108No Hit
AGTAGAAACACGAGCTGACGACAACCATGCACCACCTGTCTCCTCTGTCC60.10231923601637108No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAGG100.006912936145.04
AAGGCAC100.006912936145.05
GAAGGCA100.006912936145.04
AGAAGCG203.6747364E-4108.755
GCGGAGC258.9238817E-487.09
GAAGCGG350.003391895462.1428571
GAAGCAG350.003391895462.1428575
AGCGGAG350.003391895462.1428573
AAGCGGA350.003391895462.1428572
AAGCAGA350.003391895462.1428576
GTAGAAA400.005755712354.3751
GAGCAGA400.005755712354.3751
TAGAAAC450.00917061448.3333322
TTATACA3551.60317E-618.3802812
TATACAC3601.8332848E-618.1253
TTTTTTT400.00773859418.12590-94
CTTATAC3652.0923908E-617.8767131
TACACAT3853.4862423E-616.9480515
ATACACA3903.9437127E-616.730774
CACATCT4207.994773E-615.5357147
Produced by FastQC (version 0.11.5)