FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_99_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_99_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1600323
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA19350.073.834431
ACACGAG13250.066.199267
AACACGA13450.065.229156
AAACACG20600.063.7074135
TAGTAAC22000.063.2786452
CACGAGC14750.058.48428
ACGAGCC6700.058.425529
GCACGCA4550.054.1808932
AGCACGC4650.051.4676861
ACAACGC5250.051.088848
AGTAACA28750.048.421923
ACCGACT901.3252211E-648.3272868
ACGAGCT3000.048.3272829
GTAACAA28250.048.25234
ACGAGCA6900.047.2766889
ACACGTG8800.046.954357
AACACGT9750.044.6195566
GTAGAAA41850.043.3229681
TAACAAG32450.042.2304535
TAGAAAC42350.041.261242
Produced by FastQC (version 0.11.5)