FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_95_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_95_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003830
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA31150.15545230882859326No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA23060.1150796225228687No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG22610.1128339230373834No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC41350.086.9659962
GTAGTAA45550.079.766911
GCACGCA12950.074.460372
GTAACAA49400.072.3541264
ACGAGCG1350.069.805229
AGTAACA54750.065.681173
AAACACG30600.063.9713675
CGCACTT14700.062.1436165
TAACAAG58150.061.7161455
AGCACGC15800.061.506511
ACACGAG20550.059.2619257
CACGCAC17200.057.3262833
ACAACGC7050.055.5242778
AACACGA22450.054.9004486
ACGCACT18950.054.710434
CGATCGG400.006055061254.367527145
GTAGAAA54200.050.5784951
GAAACAC38800.050.4516454
AACACGT14450.050.1736226
CACGAGC24650.049.1108978
Produced by FastQC (version 0.11.5)