FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_94_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_94_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3098594
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT112620.3634551670854588No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC109560.3535797203505848No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT79800.2575361599486735No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT78290.25266298198473247No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT63820.2059643825554429No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT56450.18217940136720073No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC52510.16946395687850682No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG47450.1531339697940421No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT45250.14603397540949217No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA42210.13622307407811413No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC38260.12347535688767228No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT37580.12128081316881141No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC37260.12024808671287687No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG36980.11934445106393417No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT36610.11815036109925985No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC36570.11802127029226805No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC35180.11353536474930243No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG35020.11301900152133515No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT33430.10788764194341047No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC33260.10733900601369525No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT32320.10430537204938757No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA32180.1038535542249162No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAACA32000.10327264559345302No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC31790.102594918856746No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT31330.1011103745763401No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA64300.0119.5199054
AGCACGC11950.0114.685721
GCACGCA12450.0109.479772
GAAACAC24050.0109.430134
CACGCAC12600.0108.751853
AAACACG24650.0106.178265
TAACAAG74250.0103.698725
TAGAAAC34400.082.406912
TAGTAAC95050.081.69262
ACGTGCA9700.081.470452
CGCACTT17050.080.367925
ACGCACT17250.079.856424
AACACGT5850.078.078256
GAAGCGG7350.074.979361
AGTAACA103900.074.873743
AACACGA29300.073.738456
ACAAGAG113100.068.1952747
ACACGAG32450.066.793127
CGGAGCA14950.066.439252
CACAGCG554.063206E-665.910213
Produced by FastQC (version 0.11.5)