FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_92_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_92_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987822
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG33560.16882799365335527No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT31700.15947101903490352No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT29970.15076802651344035No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACT28860.14518402553146106No Hit
GTATTGGGATAGAAATGGTACATGATGGTGGGAAAACGACTTGGCACTCA28100.1413607455798356No Hit
CCCATACACTGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA27010.1358773572281623No Hit
CTTCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACA25780.12968968046434742No Hit
CTGTAGGACATTTGTCCATTGAAGGCATCAAAGAAGCAGATATAACCCCA24690.12420629211267407No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA24330.12239526476716728No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT23990.12068485005196643No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA22310.11223338910626808No Hit
GTACATGATGGTGGGAAAACGACTTGGCACTCAGCAGCAACAGCCATTTA21620.10876225336071338No Hit
CCCATACAGGCAGCAATTTCAACAACATTCCCATACACTGGTGTTCCCCC20850.10488866709393496No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA301.6009544E-596.65323145
ACAACGC6850.070.902558
CAACGCA7250.066.990699
TAGTAAC21650.061.952242
CACGAGC16200.060.4082688
AACACGA16400.060.1226736
AAACACG22250.058.652395
ACACGAG17900.055.4811177
GTAGTAA25350.054.0702251
AGTAACA25450.052.417133
GCGTAGA701.696477E-551.7863352
ACGTGCC4250.051.169369
ACGAGCC12450.050.655619
ACGAGCT2200.049.4249539
ACGAGCG1203.6288839E-948.3266149
TAGAAAC31000.048.1779982
GAAACAC27050.047.9765434
GTAACAA28050.047.3000344
TAACAAG27950.046.950475
CACGCAC3450.046.2324373
Produced by FastQC (version 0.11.5)