FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_8_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_8_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3656
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG2195.9901531728665205RNA PCR Primer, Index 17 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT1303.555798687089715RNA PCR Primer, Index 17 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT1233.3643326039387307No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT1213.3096280087527354RNA PCR Primer, Index 17 (95% over 23bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT992.7078774617067833RNA PCR Primer, Index 17 (96% over 26bp)
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGA471.2855579868708973RNA PCR Primer, Index 17 (96% over 28bp)
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTG391.0667396061269148TruSeq Adapter, Index 12 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC110.30087527352297594No Hit
ACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGC90.2461706783369803TruSeq Adapter, Index 25 (95% over 24bp)
TCTCCGAGCCCACGAGACAGGCAGAAACCTCGTATGCCGTCTTCTGCTTG90.2461706783369803RNA PCR Primer, Index 19 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAAACTCGTATGCCGT70.19146608315098468No Hit
AGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAG70.19146608315098468No Hit
CTAATAACCCGGTCGTGTTTCTACTAATAACCCGGAAGTGCGTTGTTTCT70.19146608315098468No Hit
GCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGCTTCTGC60.16411378555798686No Hit
TCTCCGAGCCCACGAGACAGGCAGAAAACTCGTATGCCGTCTTCTGCTTG60.16411378555798686RNA PCR Primer, Index 19 (96% over 26bp)
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT60.16411378555798686No Hit
AGTAGAAACAACGCACTTCCGGGTTATTAGTAGAAACACGACCGGGTTAT50.13676148796498905No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAACCTCGTATGCCGTCTTCT50.13676148796498905RNA PCR Primer, Index 19 (95% over 21bp)
AGTAGAAACAACGCACTTCCGGGTTATTAGTAGAAACACCGGGTTATTAG50.13676148796498905No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAACCTCGTATGCCGTCTTCTGCT50.13676148796498905RNA PCR Primer, Index 19 (95% over 24bp)
ATTCGATGCAACGCGAAAAACCTTACCTGGCCTTGACATGTACCGAATTT50.13676148796498905No Hit
ATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTT50.13676148796498905TruSeq Adapter, Index 12 (96% over 26bp)
GTAGTAACAAGAGCCCGGGTTATTAGTAGAAACAACGCACTCGGGTTATA40.10940919037199125No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAACCTCGTATGCCGTCTT40.10940919037199125No Hit
CTCCGAGCCCACGAGACAGGCAGAAAACTCGTATGCCGTCTTCTGCTTGA40.10940919037199125RNA PCR Primer, Index 19 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAACCTCGTATGCCGT40.10940919037199125No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGCT100.0068060136145.07
TCGATGC100.0068060136145.03
TGCAACG100.0068060136145.07
TTCGATG100.0068060136145.02
GATGCAA100.0068060136145.05
ATTCGAT100.0068060136145.01
ATGCAAC100.0068060136145.06
CAACGCG100.0068060136145.09
TCTCCGA2151.29163345E-523.6046521
CTCCGAG2151.29163345E-523.6046522
GCCCACG2201.5439775E-523.0681848
CCCACGA2201.5439775E-523.0681849
AGCCCAC2251.8380086E-522.5555577
CGAGCCC2251.8380086E-522.5555575
CCGAGCC2251.8380086E-522.5555574
TCCGAGC2251.8380086E-522.5555573
GAGCCCA2302.1794049E-522.0652186
AGACAGG1758.302881E-69.94285715-19
GGCAGAA1801.161195E-59.66666720-24
AAATCTC1706.7599074E-59.38235325-29
Produced by FastQC (version 0.11.5)