FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_89_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_89_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3367788
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT102860.3054230254398436No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC94410.2803323724652502No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT89230.26495135679561777No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG57970.1721307873298438No Hit
AAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACA52640.1563043754535618No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA50470.14986097699736445No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT49660.14745583748145666No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC47090.1398247158075271No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC42850.1272348496995654No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA39340.11681257846396506No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG38770.11512007287869663No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC35100.10422271235600342No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA23050.0116.37742
AGCACGC23400.0114.652051
CACGCAC25650.0105.428823
CGCACTT29700.094.225565
AAACACG30100.091.769085
ACGCACT31250.090.0159764
GAAACAC32900.083.958944
TAACAAG101150.080.85025
GTAACAA103400.079.090884
TAGTAAC105000.078.5761642
AGTAACA117050.070.363073
GTAGTAA119450.069.626281
GAAGCGG10400.064.1431661
ACGAGCG808.103598E-963.4280629
AACACGA34400.061.329926
AACACGT10700.060.981296
ACGTGCA11750.059.8510442
TAGAAAC58900.057.2368242
ACAACGC9050.056.869998
ACACGAG37700.055.9532137
Produced by FastQC (version 0.11.5)