FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_88_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_88_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3421023
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC72000.21046336139803795No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT64560.1887154807202407No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT63750.18634776790451277No Hit
CAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTT60770.1776369232244273No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT56560.16533066278712538No Hit
TAATATATTGTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACC54750.16003984772975802No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT51960.15188439247558408No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG49810.1455997226560593No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA49580.14492740914048224No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC48600.14206276894367562No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA47370.13846735318645914No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC47110.13770734660363287No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC45110.13186114212035405No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT43230.12636570990607196No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT40600.11867795101056029No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT39910.11666101046382908No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG39650.11590100388100283No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT39280.11481945605159626No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA37030.10824247600790758No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC35750.10450090513860913No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT35090.10257165765912711No Hit
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG35030.10239627152462874No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA34960.10219165436771399No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA34550.10099318244864183No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA34500.10084702733655987No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAAG203.862991E-4108.777411
GTAACAA54150.0107.916754
AGCACGC2900.0102.525841
GAAACAC16950.0101.802754
GCACGCA3000.0101.503332
AAACACG18350.094.035785
TAACAAG62850.093.209115
CACGCAC3300.090.078713
CGGTGCA651.6370905E-1189.2336962
CGTCAGA259.387481E-487.002862
TACGCCG259.3929336E-486.99014145
GAGCAGA7500.082.1873861
AACACGT4750.080.8973856
TAGTAAC79950.075.358952
AACACGA18150.074.299966
GCAGCGT300.001937015272.491789
TAGTAGA4100.070.7340243
ACACGAG19150.070.4097757
ACACGTG5500.069.855717
AGTAACA87950.068.6691063
Produced by FastQC (version 0.11.5)