FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_86_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_86_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3440354
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG52350.15216457376188613No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC47520.13812532082454304No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT46960.13649758135354675No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC46070.13391063826571334No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT45870.13332930274035754No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA43980.12783568202574502No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA43170.12548127314805393No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGAGGGT42140.12248739519247147No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC41730.12129565736549205No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG39890.11594737053221849No Hit
GTATTGGGATAGAAATGGTACATGATGGTGGGAAAACGACTTGGCACTCA38820.11283722547156484No Hit
CTTTACATATAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGGG36680.10661693535025756No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC35640.1035939906184073No Hit
CTATATGTGTCAGCTTCATTGTCATACTTACTATATTCGGATATATTGCT35080.101966251147411No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCCG151.2309167E-4144.980719
GTCAACG100.007094432144.9807145
GCACGCA3200.090.626112
GCGGTGC451.2317341E-680.575281
AGCACGC3600.080.575281
ACAACGC6100.071.301988
AACACGA13400.070.336686
AAACACG19550.068.236125
CAACGCA6400.067.95979
AGAAGCG9150.066.558195
GTAACAA48650.066.4653554
CACGAGC14750.063.88988
GAAACAC20950.063.6761974
ATACGTA350.00356707162.1436163
ACGAGCT954.5656634E-1061.0445069
CACGCAC5000.059.450733
AACAACG7600.057.229227
TACGTAT400.00605184454.3756644
ACGAGCC8400.053.504789
ACGAGCA8200.053.0417189
Produced by FastQC (version 0.11.5)