FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_85_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_85_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1739804
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG29520.16967428514936164No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA20070.1153578219155721No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA40200.096.142271
AGCACGC16750.095.240381
GCACGCA17350.092.763982
TAGTAAC43700.088.75612
GTAACAA49500.078.50064
CGCACTT18850.076.918725
AGTAACA51650.075.373273
ACGCACT23750.072.343264
TAACAAG54850.070.843755
AAACACG24200.069.799765
CACGCAC24450.066.1209953
CGTGCGA350.003567747562.139343
AAGAGCG10150.062.1357579
ACAACGC7700.055.545618
GAAACAC31050.053.934144
ACAAGAG72950.053.1639147
CAAGAGC70500.052.1323438
AACAAGA75050.051.872486
CAACGCA10550.050.160159
ACACGAG24300.049.222777
Produced by FastQC (version 0.11.5)