FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_84_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_84_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3091162
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG43950.14217954283858303No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG39990.12936882635073801No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT39420.1275248595835482No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGCGGGT35850.11597580456799093No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC35850.11597580456799093No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA33470.10827643455761943No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT32320.10455615072907858No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGC10300.074.600718
AAACACG16500.072.938135
AACACGA11500.069.346636
GAAACAC18150.067.505724
AACACGT6000.066.4571846
TAGTAAC41500.062.5410922
GTAACAA41850.060.6321684
ACGAGCG1200.060.4078069
ACGTGCC2450.059.1749959
TAACAAG43000.059.0106125
ACACGTG7150.055.761057
ACACGAG14350.053.5461547
TCGTATT2050.053.040997145
ACGAGCC3750.052.1923459
ACGAGCA8250.050.083569
ATTAGTA15650.050.0416071
AGAAGCG6900.049.3832055
AGTAACA53850.048.6017573
CACTCGA450.00964661748.326244145
ACCGTCT3450.048.3262448
Produced by FastQC (version 0.11.5)