FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_81_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_81_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5123885
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT187430.36579665624813984No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCCT150360.2934492089498496No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA135720.26487713912392646No Hit
CTCCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCT90410.1764481443279855No Hit
CATAATAATTGAGGGGCTTTCTGCCGAAGAGATAATAAAAATGGGTGAAA88930.17355971103957252No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTACAATTTGTTCTTTTATC87730.17121773810302143No Hit
TAATAATTGAGGGGCTTTCTGCCGAAGAGATAATAAAAATGGGTGAAACA81150.15837591983426638No Hit
GGCTTGGACAATAGGGCATTTGAATCAAATAAAAAGAGGAATAAACATGA77180.1506278927025099No Hit
TGCTAAAGCAGAGCTCCATGGGAAATTCAGCTCTTGTGAAGAAATATCTA75830.14799317314888996No Hit
GGACAATAGGGCATTTGAATCAAATAAAAAGAGGAATAAACATGAAAATA74550.14549506868323547No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA73280.14301648065871891No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG71270.13909367598999586No Hit
TTCAATACCTCCATGTTGTCAGAGAGTACTTCCCTCATTGTTTCTTTGGC70550.13768849222806523No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCC68770.1342145657055145No Hit
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG67190.13113096800572221No Hit
GTTTATTGTCTCTTTGTTTGGACCTTTTATTCGTATTTTCATGTTTATTC65980.1287694786280332No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG63150.12324632578600027No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG62110.121216615907656No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT61680.1203774089387252No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG61590.12020176096848387No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT61310.11965530061662195No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA60060.11721574547438125No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC58960.11506893694920943No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT56490.1102483759881418No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA54820.10698913031810824No Hit
CCTCCATGTTGTCAGAGAGTACTTCCCTCATTGTTTCTTTGGCTTGGATT53940.10527168349797078No Hit
GTGCTAAAGCAGAGCTCCATGGGAAATTCAGCTCTTGTGAAGAAATATCT53880.10515458485114322No Hit
AAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATCCAAG53640.104686190263833No Hit
GTCAGAGAGTACTTCCCTCATTGTTTCTTTGGCTTGGATTTCTTTTTGGT52610.10267599682662668No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT51900.10129032950583394No Hit
GGTCAGTACAGTGGGTTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA51740.10097806644762714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA33600.086.513269
GTAACAA42000.074.746774
AACAACG41600.070.3988657
ACAACGC46100.063.5269558
GAACGCG350.003569309162.134212145
TAACAAG55500.057.087675
TAGTAAC60600.053.4796752
GGAGCGT2100.051.7876056
GAAGCGG17400.050.8475951
CGTGCAC604.946112E-448.3351023
CGGAGCG2550.048.3351025
CACGAGC21300.047.6459548
AGTAACA68100.047.5898443
AAACACG32350.047.065095
ACGTGCA6900.046.2254569
GAGCAGA24950.045.924711
AGAAGCG11300.044.9131473
GAAACAC34750.044.2318344
GAGCGTT2950.044.2311367
AGCGTTT3000.043.4939468
Produced by FastQC (version 0.11.5)