FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_80_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_80_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2736623
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG28940.10575077385522229No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA27420.10019648303767087No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC33600.087.391922
GTAGTAA36950.080.275591
AGTAACA40850.071.349273
AACACGT15550.062.0121546
AAACACG40150.060.494355
AACACGA24750.060.3458756
GAAACAC40750.059.0698744
GTAACAA49700.058.3524674
ACACGAG26750.054.4698837
ACAACGC12050.053.5410778
TAGAAAC61550.052.536652
CACGAGC27050.052.2578438
AACAACG12600.051.7792937
AGAAGCG15200.051.0382545
GTAGAAA64700.050.777181
ATTAGTA21700.045.7862931
CAACGCA15750.045.105529
TAACAAG68500.042.86675
GAAGCGG28050.042.4019281
AAGCGGA28400.041.867892
Produced by FastQC (version 0.11.5)