FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_79_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_79_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3858938
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT188730.48907238209061665No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT133520.34600193110125116No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA116400.30163739350049157No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA110750.2869960595376241No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC105340.2729766583448607No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT91480.23706004087134855No Hit
TTTGTGTATCGCATGTATTCAATGATGACCAATAACCCCATAAACATCTT89140.23099619636283353No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT88810.23014103880394035No Hit
TTCTTAATCTGTGGGCCATAGCCTCAAGCATGCTATGTTGACCCACTGGC87730.2273423413384719No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC83430.21619937920743995No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA76900.19927762508752406No Hit
GATTAAGAATGGATGCACGATTAGATTATGAATCAGGGAGAATGTCAAAG74960.19425033519584922No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT72620.18818649068733417No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG71860.18621703691533784No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA65570.16991721556552608No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG63720.16512315046264026No Hit
GTTTTAACAGTGCATCATACAGGAAGCCAGTGGGTCAACATAGCATGCTT63520.16460487315422015No Hit
ATATAAATCCTTATTTTCTCTTCATAGATGTGCCCGTACAGGCAGCAATT63050.16338692147943296No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT59320.15372104967739827No Hit
GTTAAAACAGGCCTCAAATAGGTTGCAACATTTAGCGTAGCATTGTTCCT59130.15322868623439923No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT57480.14895289843993348No Hit
ATACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC56620.14672430601372707No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC56500.14641333962867503No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG55890.14483259383799377No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT54560.14138604973700017No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG52860.13698069261542942No Hit
CCCCATAAACATCTTCGAAGCTTATATGTACCCAATCTCACCAAGGTGAG52840.13692886488458741No Hit
TTCCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTA52480.13599596572943126No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT52020.13480392792006504No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG51800.13423382288080296No Hit
GTATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGT51110.13244576616675366No Hit
ATTTATATTAGCTCAAGGCCCACCCCATAGACTCAACTGTCATTCTTTGT49580.12848094475733998No Hit
GTTCATACAGTATGGCTCAAACCCTTCAATTCCAACAGACGGATTCATAA49400.1280144951797619No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC48670.12612278300402857No Hit
ACACAATAGACACCGTGATCAGAACGCATGAGTACTCAAACAAGGGGAAA47400.122831722095561No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG46520.12055130193851261No Hit
ACATAGCATGCTTGAGGCTATGGCCCACAGATTAAGAATGGATGCACGAT45840.11878915908988433No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA45520.11795991539641218No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT45480.11785625993472816No Hit
ATGTTATATTGGTCTACTGTTGTTTGCTTCAAAACTGTTTTGGCCTCATT45460.11780443220388616No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCCACTGATGAT45180.11707884397209802No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAACTCACTC43530.11280305617763228No Hit
GTATGGCTCAAACCCTTCAATTCCAACAGACGGATTCATAAATAGGAGTA43510.11275122844679028No Hit
CATGTATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATA43420.11251800365800124No Hit
ACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA43250.11207746794584417No Hit
GTAAAAGGATGTCCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG42770.11083360240563594No Hit
CACCAAGGTGAGCCATTGCTTTCTCAAAATCATCCTTTGACATTCTCCCT41840.10842361292148255No Hit
CTGTAAGATCATCAGTGGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG41660.10795716334390446No Hit
TATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATCATCCT41190.10673921166911725No Hit
GAATAAAGACTCATAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTT40940.10609136503359215No Hit
TATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATCATC40370.10461427470459489No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT40270.10435513605038485No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC40200.10417373899243781No Hit
TGACTACCCCGGTCAAGACCGCCTAAACAGACTAAAGAGAAAATTAGAGT40070.10383685874196477No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA39410.10212654362417846No Hit
GTGTATCGCATGTATTCAATGATGACCAATAACCCCATAAACATCTTCGA39240.1016860079120214No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGA100.0070942533144.98215145
GCGTCAG203.8638155E-4108.771841
GAGCAGA10450.0104.781811
GTAACAA57600.0102.960734
TAACAAG60850.097.342455
GAAACAC37400.093.630284
AAACACG38950.089.90435
TAGTAAC70050.085.2824862
AGTAACA73100.081.922553
AACACGT10850.080.1848456
CAACGCA6800.078.8873449
AACACGA33850.078.1762856
ACACGTG11500.075.642857
ACACGAG35000.075.597847
GGTGCGT300.001936113572.500473
GACCCCC608.0446625E-872.49107145
CACGAGC39800.066.1162348
ACGAGCC24250.062.4768459
CGGTGCG350.003567185562.1432572
ACAAGAG98550.060.4643947
Produced by FastQC (version 0.11.5)