FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_78_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_78_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3048046
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA82250.2698450089007843No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA52850.1733897716766742No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT51310.1683373544887446No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA43220.14179576030020544No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC41060.13470925307557693No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAATCTCAGTAAGAACAATT35950.11794441422471971No Hit
CAACAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG35880.11771475889799562No Hit
GTTTAGGTCTCTCTCTCCTTGACACCTGACACAATACCTTCCCACAATAG34660.11371219463223324No Hit
CACGAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG31690.10396824719836906No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA26150.0101.195492
GTAGTAA94300.093.892181
AGCACGC28150.093.2514651
TAGTAAC94550.093.2420962
GTAACAA94400.092.775854
CGCACTT32900.084.840815
CACGCAC31500.084.4686363
AGTAACA107450.082.047843
ACGCACT36100.078.525294
TAACAAG111950.078.490755
AAACACG44200.076.108945
GAAGCGG11800.068.827421
GAAACAC50050.067.357954
ACGTGCA15150.062.2115062
ACAAGAG142750.061.548387
CAAGAGC136050.060.3167578
CTCGCTC19450.058.894873
AACAAGA152000.058.000266
ACAACGC11650.057.8691528
ACGAGCG2650.057.4464959
Produced by FastQC (version 0.11.5)