FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_77_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_77_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4508608
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT225980.5012190015188723No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC135840.3012903317387539No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT116450.2582837097392366No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA99760.22126563231933227No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA87890.19493821596377417No Hit
GTATAGTATGGTTGTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG87620.19433936150581288No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT69870.15497022584354195No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC64870.14388032847388818No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT60950.1351858489360796No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA60310.13376634207276392No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC57620.1277999772878902No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG54080.11994832995017532No Hit
GTAAGAAGTACAGTAAAAATGAATTTAATGCGATTTTTCTACCTCCAAAA52470.1163773829971468No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT51440.1140928641389981No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA51060.11325003193890443No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGAGGGT50690.11242937953355005No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA48510.107594184280381No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC47990.10644083495393701No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA47970.1063964753644584No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT47330.1049769685011427No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT47280.10486606952744616No Hit
GCCCAAGCCATTGTTGCGAAAAATCCGCTTTTACTGCTAGCGTTAGGGCA46840.10389015855891665No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAG100.0070897667145.01313
GCACGCC100.0070942347144.98254145
CGTCATA100.0070942347144.98254145
AGCACGC32400.0123.780931
GCACGCA32750.0122.209512
CACGCAC33050.0121.10023
GAAACAC22550.0117.360944
CGGTGCA203.8655574E-4108.759832
AAACACG24950.0106.071725
AACACGT6300.092.071816
CGCACTT44700.090.511535
ACGCACT44800.090.30954
GTAACAA87550.089.691144
GCGGTGC259.3772606E-487.027171
GCGAAGC259.3855296E-487.007863
ACAACGC12400.083.59888
CAACGCA12500.082.9300169
TAACAAG102300.077.042645
AACACGA27250.075.8325356
ACACGTG7700.075.3156057
Produced by FastQC (version 0.11.5)