FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_75_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_75_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036921
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCGTCCAGGCTGGAGTGCAGTGGCGCGATCTCGGCTCACTGCAAGCT25020.12283245152855708No Hit
ATCTCGGCTCACTGCAAGCTCCGCCTCCTGGATTCACACCATTCTCCTGC21930.10766249648366333No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA28300.090.959971
TAGTAAC29650.086.316622
AGCACGC18150.084.6969151
GCACGCA19200.080.808482
CGCACTT16650.080.12135
ACGCACT19800.079.4582444
TAACAAG37400.067.460895
GTAACAA39150.065.000954
AGTAACA39100.064.898653
CACGCAC25000.062.3509143
AAACACG24800.051.452375
GTAGAAA35750.046.6509171
AACACGT10700.046.0754436
CAAGAGC54300.045.1218268
AACACGA18100.043.6608666
ACACGAG18150.043.5331047
TAGAAAC39200.042.1689872
GAAGCGG14750.041.7864341
ACAAGAG61600.041.186717
CTTAGCG3550.040.8386549
Produced by FastQC (version 0.11.5)