FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_73_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_73_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4610100
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC110480.2396477299841652No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG94490.20496301598663802No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA91940.19943168260992172No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT90150.19554890349450121No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT88570.19212164595128087No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG73110.1585865816359732No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC69110.14990998026073188No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC68060.14763237239973104No Hit
AAGCAGAGCATCTTCTCAAAAAACTGAGGCAAATAGGCCAAAAATGAACA62380.13531159844688836No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT60370.1309516062558296No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC56250.12201470683933104No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG55870.1211904297086831No Hit
GGCTTGGGCTGTCCCAAAGGACAACAACAAAAATGCAACGAACCCATTAA53530.11611461790416694No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA51890.11255721134031799No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA50830.11025791197587906No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT49920.10828398516301165No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA49750.10791522960456389No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCGGGGCC49020.10633174985358236No Hit
GCCCAAGCCATTGTTGCGAAAAATCCGCTTTTACTGGTAGCGTTAGGGCA48490.10518210017136287No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT48410.10500856814385806No Hit
TCCCTATAATGCACGACAGAACAAAAATCAGGCAACTAGCCAATCTTCTC48240.1046398125854103No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT48120.10437951454415305No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT47710.10349016290319081No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA47120.10221036420034273No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG46520.10090887399405651No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCTTCA46390.10062688444936119No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC100.0070875715145.028151
CGGTGCA202.1310989E-6145.004552
CCGGAGA100.0070912503145.002994
TCGTGAC100.0070942403144.982549
AGCACGC14300.0117.138121
GCACGCA14450.0115.903292
CACGCAC14650.0114.319753
GAAACAC17900.0108.549724
AAACACG19750.097.6475755
AACACGT5150.078.836576
AACACGA19700.077.285856
ACACGAG19950.076.3065957
CAACGCA10100.074.644489
ACACGTG5450.074.4864357
ACAACGC10050.074.294538
CGCACTT24050.074.159535
ACGCACT24400.073.0957644
GCGGTGC406.659329E-572.514081
TCTCGTT502.3092161E-672.49127145
CGAGGAT851.6916601E-1068.2366945
Produced by FastQC (version 0.11.5)