FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_6_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_6_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4015411
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT64650.16100468918374733No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA62900.15664648027312772No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC60620.15096835666386332No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA57860.14409483861054323No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG57560.14334771708300842No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG57050.1420776104861993No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC56970.1418783780788567No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT56390.14043394312562277No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA54870.13664852738611313No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG51840.12910259995801177No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT51330.12783249336120261No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGCGGGT49170.12245321836295213No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT47920.11934021199822384No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG47800.11904136338720993No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT47380.11799539324866123No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA47300.11779616084131861No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG46560.11595326107339947No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC46120.11485748283301511No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT45830.11413526535639813No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC44810.11159505216277986No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC44750.11144562785727288No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC43940.10942839973292896No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG43570.10850694984896939No Hit
AATGATAACTGATGGCTCAGCTTCAGGTGTTAGTGAATGCAGATTCCTTA43390.10805867693244851No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC42320.10539394348424108No Hit
GTCTACACCTGTGACAGTGTCCCACAGCAGCTGTCCTGACCCCATTAAAC42190.10507019082230935No Hit
GTCTGGGGGTGTCCAAATAAGTATCTGTGCACATCAATCTTATTTCTGCT41960.10449739765119935No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT41220.10265449788328018No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGAC100.007094753144.9788145
GTAACAA51050.085.918814
AAACACG15900.076.146165
AACACGT5200.073.892636
TAACAAG59550.073.533255
ACGCGAA300.001937291772.48941145
AACACGA11700.070.63956
TAGTAAC67300.067.219952
ACGAGCT554.0673604E-665.899469
ACACGTG5900.065.11767
AGTAACA70100.064.534983
CACGAGC12800.063.4282268
GAAACAC21750.055.6654624
ACACGAG15500.052.847127
GCGTTAA450.00964680648.3262679
ACGTGCA4950.048.3262679
CACGTGC8150.047.1403478
ACGTGCC1850.047.0201539
CAACGCA5100.046.9049079
GGAGCAT15350.046.7579463
Produced by FastQC (version 0.11.5)