FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_67_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_67_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2409232
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA58470.24269144690092112No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA43150.1791027182106165No Hit
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA29330.12174003997954534No Hit
AGCACGCACTTGAGGGGAAAGCAAAAGGAGCAAGGAGGCGGTGGAGGCAG28170.1169252276244048No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC26730.11094821918354064No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC23800.0101.162621
GCACGCA24450.098.452782
TAGTAAC65950.095.097722
GTAGTAA66300.094.615331
CGCACTT29000.084.005955
AAACACG37800.080.369455
GTAACAA78900.079.397264
CACGCAC30750.078.753553
ACGCACT32550.078.630774
AGTAACA80000.078.396183
TAACAAG83250.075.335675
AACACGT14900.067.639026
GAAACAC44700.067.6390154
AACACGA33600.060.6367386
GAAGCGG12550.059.5185661
CGGGTAC1350.059.068455145
CGGAGCG1600.058.9104235
ACAAGAG109850.057.1497737
CAAGAGC104750.057.0255978
AACAAGA113900.055.1904756
Produced by FastQC (version 0.11.5)