FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_66_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_66_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2161928
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT73160.3384016488985757No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC68110.31504286914272817No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT67480.3121288035494244No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT59660.2759573861849238No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT54910.25398625671160185No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT47120.21795360437535388No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC43270.20014542574960867No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG38670.1788681214175495No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT33690.15583312672762462No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC29330.1356659426215859No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAATCTTCTC29170.1349258624709056No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG28140.13016159650090106No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC28120.13006908648206603No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT26230.12132688970215476No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG26130.12086433960797954No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT25650.11864409915593858No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC25300.1170251738263254No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT24900.1151749734496246No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT24480.11323226305408876No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC24450.1130934980258362No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA24100.111474572696223No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA23930.11068823753612517No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAACA23570.10902305719709446No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG23510.10874552714058931No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA23340.10795919198049149No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT23250.1075428968957338No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA23190.10726536683922869No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA22760.10527640143427534No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT22760.10527640143427534No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC22740.10518389141544028No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA22640.1047213413212651No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC22410.10365747610466212No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT22380.10351871107640957No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA22320.10324118101990445No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAGCA22020.10185353073737886No Hit
GTTTTCAACTACTAATACAAACCCTCTTATTTGTATTCCAGCAGTGGCAA21670.10023460540776565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA43700.0125.09684
AGCACGC9350.0122.5497061
GCACGCA9500.0120.584032
CACGCAC9800.0116.892673
GAAACAC17450.0114.6753164
TAACAAG48250.0113.149855
AAACACG19350.0103.7898945
GAGCAGA4250.0102.383311
CGGTGCA451.0919393E-896.670742
TAGAAAC25100.087.52362
TAGTAAC63500.087.232022
CACAGCG502.2691893E-887.003673
ACGCACT14100.083.3013844
CGCACTT14250.082.424525
AGTAACA67850.081.532543
CAGCGTT554.395588E-879.094245
CAGCCTT2200.079.094245
AACACGT4850.077.735246
AACACGA21250.077.450326
ACGTGCA7050.075.074082
Produced by FastQC (version 0.11.5)