FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_65_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_65_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2207660
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA47610.21565820823858747No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT47130.21348396039245177No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC46020.20845601224826288No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT40740.18453928594076985No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA30770.13937834630332568No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG30160.1366152396655282No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC27890.12633285922651133No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT25220.11423860558238134No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA24900.11278910701829085No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC20700.0114.901321
GCACGCA21650.0111.1765442
CGCACTT24600.0103.738645
GTAACAA75300.097.435794
CACGCAC24900.097.247873
TAGTAAC77200.095.0377662
ACGCACT26900.094.868794
TAACAAG80300.091.45915
GTAGTAA84350.087.7732541
AGTAACA85300.086.098063
AAACACG27850.083.823095
GAAACAC30900.075.783924
GAAGCGG7500.070.580241
GTCTCGC13100.069.746241
ACAAGAG106250.069.181957
CTCGCTC13600.065.036013
CAAGAGC98350.064.788568
AACAAGA115200.063.940236
CGCTCTG13500.063.369645
CGGAGCG1150.063.0427475
Produced by FastQC (version 0.11.5)