FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_64_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_64_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3468513
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAATATATTGCTAAAATTCTCACCAACAGAAATAACTGCACCAACAA114650.33054510679360294No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC111340.32100211243261884No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT110710.3191857721161777No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT88240.25440296749644586No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT70960.20458334738834766No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC67220.19380062868439588No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT61150.17630033388947944No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC49210.14187636027311992No Hit
ATTCGAATATATTGCTAAAATTCTCACCAACAGAAATAACTGCACCAACA46580.13429386022194525No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT44110.12717265294954927No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA43680.12593292860658156No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC42950.1238282803034038No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC41450.1195036605023536No Hit
ATATCCGAATATAGTAAGTATGATAATGAAGCTGACACATATAGTGATAA41240.11889821373020656No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG40140.1157268258761031No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT39520.1139393163583357No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA38820.11192116045117893No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG38760.11174817565913692No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA38300.11042195892014821No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC38250.11027780492677985No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA37890.10923989617452781No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA37190.10722174026737107No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC36930.10647213950185569No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT36870.10629915470981369No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG36320.10471346078276195No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT36100.10407918321194126No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC35490.10232050449284752No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCA35340.1018880425127425No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT35040.10102311855253246No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG34970.10082130296181677No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA69050.0121.0634164
AGCACGC8500.0114.316341
GCACGCA8850.0109.777942
GAAACAC26600.0109.297454
AAACACG27350.0106.5653465
CACGCAC9150.0106.177143
CGGTGCA501.9463187E-10101.503632
TAACAAG85950.097.343555
TAGAAAC33900.092.606552
TAGGACG259.387919E-487.001864
ACGCACT11950.086.152474
AACACGT8200.084.879876
CGCACTT11950.084.332355
TAGTAAC101350.082.91122
ACGTGCA10750.073.514262
AACACGA30650.072.619836
AGTAACA116100.072.5639953
GCTGCGT300.001934653872.514091
ACACGTG9650.072.112387
GAAGCGG8050.069.3613051
Produced by FastQC (version 0.11.5)