FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_63_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_63_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2164165
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC36990.17092042427448922No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG36780.16995007312289034No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA32840.15174443723098746No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC26100.1206007859844328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA10600.096.44312
AGCACGC11000.092.964021
GTAACAA47750.081.841454
CACGCAC13350.077.1196443
AAACACG19550.073.430485
TAGTAAC53550.073.383392
TAACAAG54350.071.636225
CGCACTT14050.069.6651155
AACACGA14550.067.769436
ACGCACT16050.067.760124
AGTAACA62100.063.3966263
GTAGTAA64800.062.00451
AACACGT7300.061.5781676
ACGAGCC5450.058.5239759
CACGAGC17000.057.5655338
ACACGAG17300.056.98637
GAAACAC25200.056.6791884
ACAACGC7050.055.52428
ACAAGAG75450.051.9775357
ACACGTG9100.049.388747
Produced by FastQC (version 0.11.5)