FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_61_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_61_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815800
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT45940.25300143187575724No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC28130.15491794250468113No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG24860.1369093512501377No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA20150.110970371186254No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC18980.10452693027866505No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT18500.1018834673422183No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA12350.0101.558382
AGCACGC13000.095.941261
CACGCAC14150.088.639293
AAACACG16050.081.7598655
GTAACAA47800.079.7800754
TAGTAAC51900.074.315752
CGCACTT17150.073.9793555
TAACAAG52300.073.3315055
GAAACAC17800.072.907074
CGGAGCG809.822543E-1172.499765
ACGAGCG406.669306E-572.489789
AACACGA13450.071.691226
ACGCACT18600.070.550854
AACACGT5200.068.317096
AGTAACA57100.067.547943
GTAGTAA58400.066.926211
ACACGAG15050.063.0974167
ACAACGC7600.062.9516538
ACACGTG6200.057.2903147
AAGAGCC22050.056.8740739
Produced by FastQC (version 0.11.5)