FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_5_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_5_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1730083
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA29080.16808442138325155No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT25780.14901019199656895No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC25220.14577335307034403No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG25210.14571555237523287No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA24580.14207410858322983No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGCGGGT24440.1412648988516736No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC23670.13681424532811431No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG23430.13542702864544648No Hit
TAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCCCTTGAAGAGAGAAAAG21690.12536970769610475No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT21300.12311548058676955No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA19480.11259575407653853No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA19450.11242235199120504No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG18710.10814510055297925No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT18620.10762489429697882No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT18410.10641107969964446No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGCA100.007093119144.98761145
AACACGA6350.084.493176
AAACACG8400.081.9988865
ACACGAG7150.075.028557
CACGAGC7150.074.014658
GAAACAC9400.073.2756044
AACACGT2150.070.818136
GTAACAA25450.069.228054
TAGTAAC26550.067.998362
ACGAGCA5050.064.5984349
ATCTCGG350.003568151562.137543145
TTCGGAC350.003568151562.137543145
TAACAAG28800.061.4272355
GAAGCGG6000.058.015161
CAACGCA4900.051.781299
AAGCGGA6850.050.805922
ACGAGCC4000.050.745669
AGTAACA37100.048.857333
AGAAGCG4100.047.7467165
AACAACG5200.047.3997967
Produced by FastQC (version 0.11.5)