FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_57_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_57_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3660110
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC70330.192152694864362No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT51200.13988650614325798No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA43310.11832977697391608No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC43100.11775602372606286No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT42830.11701834097882305No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG42480.11606208556573436No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA42180.11524243806880122No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC42160.11518779490233899No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT40970.1119365264978375No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC36950.10095325003893327No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA68500.093.0351264
AGCACGC11050.092.535071
TAACAAG75450.084.4652945
GCACGCA12300.083.111792
AAACACG29000.082.752035
CACGCAC13350.076.574913
GAAACAC32350.075.303234
TAGTAAC90300.072.4214862
AACACGA25750.069.5453956
CGCACTT15150.068.434025
ACGCACT16400.066.312614
AGTAACA101600.064.366733
AACACGT9850.063.3010446
ACACGAG28550.061.6997687
ACGAGCG606.8082136E-660.4181485
ACAAGAG110400.058.1108977
CACGAGC30250.057.753078
GAAGCGG9450.056.7870671
ACGAGCA22450.051.663889
CGGAGCA11750.050.5969852
Produced by FastQC (version 0.11.5)