FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_56_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_56_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3198505
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC57620.1801466622687787No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA52480.16407665456205323No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT51690.16160675065382107No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA34730.10858197814291365No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA86500.095.717744
GCACGCA18700.090.335152
TAGTAAC93650.088.564712
AGCACGC19200.088.001941
TAACAAG96550.085.52915
AAACACG36500.084.418675
CGCACTT22050.079.5699845
CACGCAC21800.077.82193
AGTAACA108800.076.2990343
ACGCACT24650.073.530164
GAAGCGG11550.070.3191451
GAAACAC46700.065.669844
GTAGTAA129150.065.1331
AACACGA34800.065.000666
ACAAGAG128800.064.274457
CAAGAGC115750.060.4984558
ACACGAG39650.056.8598027
TAGAAAC70100.056.1596372
AACACGT15200.054.3755576
AAGCGGA15750.051.095762
Produced by FastQC (version 0.11.5)