FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_53_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_53_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1272260
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA17730.13935830726423845No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA15160.1191580337352428No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG14010.11011900083316302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC24550.086.538612
GTAGTAA26200.082.765451
AGTAACA30450.069.532743
GTAACAA32300.065.101244
TAACAAG32550.064.6012345
AAACACG14200.061.7861065
AACACGA10850.055.4679576
ACACGAG10900.054.5332877
TAACGCT400.006048186754.381994
CGGAGCG400.006048186754.381995
CACGAGC11150.052.660448
ACGAGCC5100.051.1689649
CAAGAGC42550.047.020138
CAACGCA7300.044.685239
AACAAGA47200.044.5502136
GTAGAAA31500.044.4350781
ACAAGAG47800.043.827267
TAGAAAC32300.042.6525342
AACAACG7500.042.5270967
AACACGT7000.041.43396
Produced by FastQC (version 0.11.5)