FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_51_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_51_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4027919
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG99390.24675272764919054No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA92800.23039192198254238No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA89960.22334113471497316No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG87020.2160420802900952No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGGAGTA79830.19819167167959437No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC78660.1952869459390817No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG72900.1809867576780963No Hit
CATATGACCAGAGTGGAAGACTTGTTGCTAAACTTGTTGCTACTGATGAT69730.17311668879140815No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT68860.17095676452282182No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAATTCACTC67740.16817617236096358No Hit
CTTCAAGAGACATCCTTTTACTTTCAGGCTCACTTTTGTTGTGAGTCTTT66840.1659417679451846No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT65020.1614233056821649No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA64350.15975991572819612No Hit
CCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAATGGATAC62890.15613521523148802No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC61810.15345392993255325No Hit
TGACTATCCTGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT57230.14208329412781143No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG55550.13791240588502401No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG52680.1307871384702622No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGTCTTCCACTCTGG51410.12763414557244074No Hit
ATATGTTATCATCAATCCTTTTACTTAAAAGATAAAGTTCTTCCGTGACT50340.12497768698923688No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTT49680.12333912375099897No Hit
GGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGTCAAGAATAAA49360.12254466884761088No Hit
AGATAAAGGAGGAAGTAAACACTCAGAAAGAAGGGAAGTTCCGGTTGACA46560.11559318844296522No Hit
ATTATGGAGCTGTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATC46530.11551870829577257No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA46210.1147242533923845No Hit
GTAAAAGGATGTCTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG46120.11450081295080661No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC46020.11425254579349783No Hit
TTATGAATCCGTCTGCTGGAATTGAAGGGTTTGAGCCATACTGTATGAAA45780.11365670461595677No Hit
GTTTAGTCACTGGCAAACAGGAAAAATGGCGGACAACATGACCACAACAC45560.11311051686987747No Hit
GTTTAGGCGGTCTTGACCAGGATAGTCAAGGGCCCTTTGCCATGAAAGCC45340.11256432912379816No Hit
TTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGGGAGTCCTATCCCAAT43050.10687901122142723No Hit
CAATTGGACCGATTACCCTTCAACACCAGGGAGGTGCCTTGATGACATAG42770.10618386318096268No Hit
GTTATCATCAATCCTTTTACTTAAAAGATAAAGTTCTTCCGTGACTAGTC42730.10608455631803916No Hit
CCTTTATCTTTTGCCTTGCATCTTTGTTGTTCATGTCCCTTAATACTATT42530.10558802200342163No Hit
CCTTAATACTATTTCAGTTGGGCCATCAACATCATCTGGTTCTTCTTCTA42410.1052901014146511No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCAT42260.10491770067868793No Hit
AATTGGGATAGGACTCCCACCGCAGTTTCAGCTGCTCGAATTGGCTTTGA42240.10486804724722618No Hit
GTCCGGGAGCAACCAATGCCACCATAAACTTTGAAGCAGGAATTCTGGAG40610.10082129258309315No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA4500.074.114922
ACAACGC14550.068.754488
CAACGCA15150.067.945489
AGCACGC5250.066.303941
ACGTGCA3350.056.2715532
AGCTCGC553.2221203E-452.7208188
GTAACAA78850.050.572684
CGTGCAG901.3242261E-648.335223
AACAACG20550.048.3274157
CACGCAC7000.047.644723
TAGTAAC84300.047.303742
TAACAAG87500.045.656075
AGTAACA91400.043.6286163
ACACGTG7100.042.882087
ACGAGCA11850.042.2100269
GTAGTAA103050.040.8166541
GAAACAC24900.040.1822934
GAAGCGG6200.038.599321
ACACGAG18500.038.4007077
AAACACG26100.038.334835
Produced by FastQC (version 0.11.5)