FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_50_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_50_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194147
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA84640.3857535525194985No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC66570.3033980859076443No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC49520.22569135067067067No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT44980.204999938472673No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT38830.17697082283001092No Hit
ACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA37550.1711371207125138No Hit
CTTCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACA36740.16744548109128513No Hit
GTACAATGGTAGACCCAACAAATGGACCATTACCTGAAGACAATGAGCCA36540.1665339651354262No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA36170.1648476606170872No Hit
CCCATACACCGGCGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA36000.16407287205460708No Hit
CCCATACAGGCAGCAATTTCAACAACATTCCCATACACCGGCGTTCCCCC32760.14930631356969246No Hit
GATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACCGGCGT32320.14730097846680282No Hit
GATATAACCCCAGCACATGGTCCCGTGAAGAAAATGGATTATGATGCAGT29470.13431187609581308No Hit
TTCCCATACACCGGCGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCA29000.1321698135995446No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA28810.13130387344147862No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGC28410.12948084152976078No Hit
GTAGAAACACGAGCCTTTTTCATTTTAATCATTTGTTTGTCACATGTATC27500.12533344393060264No Hit
GTTTTAACAGTGCATCATACAGGAAACCAGTAGGTCAGCACAGCATGCTT27020.1231458056365412No Hit
ATACTAAATACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGC26460.12059356096013622No Hit
ATCTGGGACTCATAGCTGGAGAACTAAAAGGAACAGATCTATACTAAATA26300.11986434819544907No Hit
GATTAAGAATGGATGCACGACTAGATTATGAATCAGGAAGAATGTCAAAG24360.11102264342361748No Hit
ATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATA24230.11043015805230916No Hit
GTAGTAACAAGAGCCTTTTTCATTTTAATCATTTGTTTGTCACATGTATC23740.1081969439604548No Hit
ATGGTAGACCCAACAAATGGACCATTACCTGAAGACAATGAGCCAAGTGC22730.1035937883833672No Hit
GTCCCAGATACTGCATCATAATCCATTTTCTTCACGGGACCATGTGCTGG22290.10158845328047757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG13600.079.433625
ACACGAG10400.071.0926747
TAGTAAC27750.069.237262
AACACGT5050.068.91396
GCACGCA2350.067.8753052
TTGGTCG754.8657967E-967.6542059
TAACAAG28250.066.985235
GAAACAC16450.065.671564
TAGAGCG350.003569675162.1314139
AACACGA12250.060.3699846
ACAACGC7700.060.248648
CACGAGC12400.059.6261148
CAACGCA8050.056.7286839
CGGAGCG2850.055.9673585
GTAACAA35500.053.5093844
AGTAACA36400.052.5847133
ACGAGCC7450.050.5947049
GTAGTAA40050.050.341711
GAGCGCG604.950877E-448.3244329
ACACGTG7100.047.9841167
Produced by FastQC (version 0.11.5)