FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_4_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_4_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3413109
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA82210.24086543969149538No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG79770.23371653234631534No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA73160.2143500251530203No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT69900.20479861615905032No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT65860.19296190071867028No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG63920.18727793340324025No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT63760.18680915259372027No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC60000.17579280357000027No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCATAAATAGGAGTA58450.17125148947777524No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG57280.16782352980816026No Hit
TCTTAATGAAGGACATTCAAAGCCAATTCGAGCAGCTGAAACTGCGGTGG55490.16257904450165522No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG55140.16155358648083024No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA54980.16108480567131023No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT54080.15844791361776023No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC53360.15633839997492022No Hit
TGACTATCCTGGTCAAGACCGCCTAAACAGACTAAAAAGGAAATTAGAGT52750.1545511731386252No Hit
CATATGACCAGAGTGGAAAACTTGTTGCTAAACTTGTTGCTACTGATGAT52660.15428748393327021No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA51480.1508302254630602No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC50570.14816403460891522No Hit
CCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAATGGATAC50100.14678699098095022No Hit
ATCTTACAGTGGAGGATGAAGAAGATGGCCATCGGATCCTCAATTCACTC48490.1420698840851552No Hit
ATATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACT46570.1364445143709152No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA46430.1360343311625852No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGACGGATTCAT45920.13454009233224018No Hit
GGTCAAGACCGCCTAAACAGACTAAAAAGGAAATTAGAGTCAAGAATAAA42650.12495938453767519No Hit
ATTATGGAGCTGTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATC40750.11939261242462516No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGTTTTCCACTCTGG40560.11883593521332017No Hit
ATGCAGAGTTGATAAGGACTTGTATCCATTGGGGTGTTTGAGGAATGTTC39160.11473410313002017No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA38120.11168702786814017No Hit
GAGCCATACTGTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAA37710.11048577704374515No Hit
GAATAAAGACTCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTT37280.10922592861816016No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG37020.10846415980269015No Hit
TTACTGTATTGTCTCATGGTCATGTACCTGAACCCTGGAAATTATTCAAT36570.10714571377591516No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT36280.10629604855866015No Hit
GTATTGTCCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAACACCCCAA36140.10588586535033016No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA36060.10565147494557015No Hit
GTAAGAAGTACAATAAAAATTGAATTTAATGCGATTTTTCTACCTCCAAA35880.10512409653486016No Hit
CTTCAAGAGACATCCTTTTACTTTCAGGCTCACTTTTGTTGTGAGTCTTT35500.10401074211225014No Hit
GCGTAATGTATTGTCCCTTAGAGTGTTAGTAAACGGAACATTCCTCAAAC35130.10292668649023516No Hit
GGATACAAGTCCTTATCAACTCTGCATAGATTGAATGCATATGACCAGAG34350.10064138004382515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA256.4674477E-6115.998852
AGCACGC11400.0108.772821
GCACGCA11950.0103.743732
CACGCAC12300.0100.791683
GAAACAC8800.087.3286744
CGAGGAT801.8189894E-1281.5616765
AAACACG10000.076.124245
GTAACAA50150.075.318294
CAACGCA11000.075.126819
ACAACGC10850.074.8291858
ACACGTG2750.073.808797
ACGAGGA754.8603397E-967.6659854
AACACGT2900.067.499336
TAACAAG58950.064.3208165
CACGAGG808.094503E-963.4368633
AACACGA9250.061.134526
ACACGAG9250.061.127367
CGCACTT21050.060.6169745
AGTACAG606.8095087E-660.416065
ACGCACT22050.059.5118754
Produced by FastQC (version 0.11.5)