FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_49_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_49_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4331556
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG74470.17192436159200064No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC74230.1713702881828147No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA71950.16610659079554785No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT70950.16379795159060623No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG61770.1426046436892424No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC58970.1361404539154059No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA56950.1314770027214239No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA53330.12311972879953531No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG52420.12101886712303847No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG49480.11423146786051018No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC48420.11178431030327209No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC48270.11143801442253085No Hit
GGCTTGGGCTGTCCCAAAGGACAACAACAAAAATGCAACGAACCCATTAA48050.11093011379744368No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT45410.10483530629639788No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT45090.10409654175081656No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC45030.10395802339852007No Hit
GCCCAAGCCATTGTTGCGAAAAATCCGCTTTTACTGGTAGCGTTAGGGCA44070.10174172976177615No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT43770.10104913800029365No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA43750.10100296521619483No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA203.868766E-4108.73706145
AGCACGC7400.0107.7985151
GCACGCA7400.0107.774872
CACGCAC7950.0100.318753
GCGAAGC451.0921212E-896.670793
GAAACAC21850.091.914674
AAACACG22150.090.669785
AACACGT5200.086.4466
ACAACGC7100.083.7224358
ACACGTG5450.082.4672557
CAACGCA7250.081.990249
AGCGAAG554.3966793E-879.0942842
AACACGA20600.076.022666
ACACGAG20600.076.0103767
GTAACAA80550.069.667784
ACGAGGA1500.062.825869
CACGAGC23500.062.620218
TAACAAG90900.061.8948335
TAGTAGA5750.061.7852443
GAAGCGG12050.058.9781071
Produced by FastQC (version 0.11.5)