FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_48_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_48_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3122562
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT145070.46458645176620994No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC114160.3655972243305337No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT94880.30385305399860757No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA75440.2415964839128895No Hit
GTATAGTATGGTTGTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG67810.21716142065393737No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA62660.20066855357875998No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC59780.19144535801050547No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT55300.17709816490433175No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC36470.11679511887994537No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG35830.1147455198647777No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT35620.11407299518792582No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGG34750.11128682152668225No Hit
GTAGAAACACGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC34380.11010189709603845No Hit
GCTGTAGACATAGGAAATGGATGCTTCGAAACCAAACACAAATGCAACCA34280.10978164724991848No Hit
GATATACAGAAAGCACTAATTGGTGCATCTATCTGCTTTTTAAAACCCAA32490.10404917500437141No Hit
GCGGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG32290.1034086753121315No Hit
AATTAGGAGTGCTTTCTGAAAGGTTGAGCCCGGGCAAGATAAACGAGGGT31880.10209565094303971No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGC100.0070909197145.00454
ATCGACT100.0070909197145.00453
AGCACGC26850.0120.723341
GCACGCA27300.0118.978052
CACGCAC29100.0111.369443
AAACACG23300.0102.374425
GTAACAA74300.0101.190894
GAAACAC24250.098.961844
CGCACTT35400.095.440825
ACGCACT36300.093.074524
TAACAAG88500.084.954625
TAGTAAC95050.080.015632
AACACGT6300.077.105576
AGTAACA102900.073.840973
AACACGA26500.071.955066
ACAACGC9250.068.961858
GTAGTAA113450.068.456231
ACACGAG29700.064.1899957
ACGTGCA9550.063.0124322
GAAGCGG7600.062.9735761
Produced by FastQC (version 0.11.5)