FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_46_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_46_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145514
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA41400.1929607543926537No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA23740.11064947606960382No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA21760.10142091825082475No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG100.0070941946144.98106145
AACACGT7500.080.237816
TAGTAAC37200.076.988622
AAACACG26150.069.038275
GTAGTAA42800.068.959461
AACACGA18900.064.831666
GAAACAC29300.061.8635184
ACACGAG20250.060.498277
CACGAGC20250.060.498278
TAACAAG47600.059.861535
AGTAACA48400.059.6210983
CAACGCA10850.059.4622769
ACGAGCC11900.055.4339339
TAGAAAC42150.054.013762
ACGAGCA6050.053.9185759
GTAACAA54500.052.4157684
ACAACGC10550.049.472218
AACAACG12150.043.5539787
GAAGCGG13200.042.8512881
AGAAGCG9150.042.789275
Produced by FastQC (version 0.11.5)