FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_42_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_42_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3684029
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT81950.22244667455115036No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC78290.2125118993362973No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT76690.2081688282041211No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT75540.20504724582786943No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC63520.17241992394739564No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT48040.13040071074359078No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT47180.12806631001004606No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC45740.12415754599108747No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC45620.12383181565617425No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA45260.12285462465143461No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC44310.12027592616670499No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC44020.11948874452399805No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT43540.11818582318434519No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA42640.11574284567249607No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC42450.11522710597555014No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG42140.11438563594369099No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC41180.11177979326438528No Hit
CCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT40920.11107404420540663No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA40750.11061259289761292No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT40520.10998827642236257No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT40140.10895679702847072No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT39570.10740957793763295No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG39450.10708384760271975No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT39150.10626952176543669No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG39040.10597093562509957No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA38910.10561806109561027No Hit
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG38180.10363653489155486No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA38040.10325651616748945No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA37250.10111212479597745No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG37190.10094925962852083No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCACG256.4642954E-6116.010474
CACGCAC5450.0114.4139943
GCACGCA5500.0113.373872
AGCACGC5550.0112.3768841
GAAACAC19300.0111.953114
GTAACAA60350.0110.652074
GAGCAGA6000.0106.3660351
AAACACG20700.0104.731675
CGTGCAC301.5993151E-596.67543
TAACAAG74100.090.413025
AACACGT5400.081.905546
ACGTGCA8700.080.840632
AACACGA21850.078.977386
TAGTAAC90900.074.4209062
CGCACTT9000.074.1178055
GTTAGGT300.001935462972.5065463
CGGTGCA406.662579E-572.5065462
ACACGTG6100.072.491787
TAGAAAC32450.071.9479452
TAGTAGA3150.071.355653
Produced by FastQC (version 0.11.5)