FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_40_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_40_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1993920
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC57220.28697239608409564No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT55700.27934922163376663No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT49970.25061186005456587No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC47620.23882603113464934No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT45920.2303001123415182No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA32390.16244382924089232No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC31070.15582370406034346No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT30780.15436928261916225No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC30430.15261394639704703No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC29230.14659565077836623No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG28070.14077796501364145No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT27430.13756820735034506No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA27000.13541165142031777No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG26620.13350585780773552No Hit
AGCATATACTGACACATACCATTCTTATGCAAACAAAATCCTAAGAACAC26040.13059701492537315No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG25200.12638420799229658No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTACTGTCAGGGCC25140.12608329321136252No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA23890.11981423527523671No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA23720.1189616433959236No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA23630.11851027122452254No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT23460.11765767934520943No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC23320.11695554485636334No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCA22400.11234151821537473No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT22300.11183999358048467No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG22300.11183999358048467No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT22210.11138862140908361No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA21640.10852993099021023No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT20390.1022608730540844No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAACA20140.10100706146685925No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTCG100.0070863236145.034654
GCGATCC100.0070932345144.987379
AGCACGC2500.0121.853561
GCACGCA2600.0117.143372
GTAACAA43750.0112.3811344
CACGCAC2800.0108.7759863
AAACACG10950.0101.325575
GAAACAC11500.095.8489764
TAACAAG51600.095.424935
CGGTGCA502.266097E-887.020782
AACACGT3500.080.8050166
TAGTAAC61900.080.600842
ACGTGCA5350.077.261442
CGGAGCA9350.076.0074542
CGTGCAC300.001934191472.517323
AGTAACA68950.072.3595663
GAAGCGG4350.071.698171
ACAAGAG69450.070.980137
ACACGTG4050.069.808737
GGAGCAT10450.067.312733
Produced by FastQC (version 0.11.5)