FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_39_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_39_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1716500
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCTAAAAACAATTTTAGGTAGAAAACAGAGTGTGAGTT32200.1875910282551704No Hit
ATATATATCTTCACACAAATATGAAAGTCAGCTCTAGAAATAACAGAATG24920.14517914360617534No Hit
CTTTATAGCAACGCAAAAAATGGATGAACACAGCTGTGTCCTGTGAACTC19960.11628313428488203No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA27600.084.079391
TAGTAAC28300.081.46822
AGTAACA33550.068.503723
AAACACG26850.056.705145
AACACGT10950.054.955536
AACACGA17300.053.6427846
ACAACGC10100.052.3960238
ACACGAG18450.049.900427
CACGAGC18650.048.9765938
CAACGCA18150.047.1305169
GTAACAA48700.046.7463954
TAACAAG48700.046.5975235
ACGAGCT6200.044.431279
ACACGTG13400.044.3614657
CGGAGCG1801.8189894E-1244.3065155
AACAACG12100.043.7355237
ACGAGCC7300.043.694499
AGAAGCG4700.043.192435
ACGAGCA6750.040.8109449
TAGAAAC53900.039.8153342
Produced by FastQC (version 0.11.5)