FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_38_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_38_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3744368
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT87410.2334439349978421No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC77070.20582912790623142No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT67600.18053781038615863No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT65320.1744486653021284No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC56310.1503858595095354No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA51400.13727283215752298No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA46190.12335860150498028No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC44360.11847126137174552No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT42560.11366404156856377No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT42560.11366404156856377No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC42530.11358392123851074No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC41340.11040581481307392No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG41220.11008533349286181No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT40890.10920400986227849No Hit
TACCATTCCTATGCAAACAAAATCCTAAGAACACAAGAAAGTGCCTGCAA40750.10883011498869768No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA40100.1070941745042154No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA39450.1053582340197331No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA39320.10501104592283664No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG38500.10282109023472051No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC38290.10226024792434933No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG37490.10012370578960188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA55650.0115.953384
AGCACGC9700.0112.145651
GCACGCA9800.0110.974632
GAAACAC21100.0104.4599154
CACGCAC10550.0103.085443
AAACACG21900.0100.644035
TAACAAG71650.089.958895
GAAGCGG7550.085.488171
ACGTGCA9200.083.536552
AACACGT5300.076.607396
TAGTAAC88500.074.3876952
AACACGA24300.074.293636
CGCACTT15850.072.732155
ACACGAG25850.070.1062557
ACGCACT16550.070.093954
TAGAAAC36500.067.5374452
CAACGCA8450.066.0558559
AGTAACA104100.063.309913
GTAGAAA39600.062.265391
AAGCGGA10250.062.246842
Produced by FastQC (version 0.11.5)