FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_37_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_37_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550562
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCGGGGGTTGGAGGAGTTGAGGGTGAAAGTCCGGGCAA21050.13575722866934697No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTCG100.0070947357144.97607145
GTAGTAA19150.082.9242861
CGGAGCG755.638867E-1177.328055
AAACACG21150.066.153885
TAGTAAC24200.065.305462
TAACAAG24850.064.180735
ACACGAG17000.055.0056277
CGCACTT5650.051.323935
AACACGA18650.049.7553186
GTAACAA32700.048.3300324
CACGAGC19200.046.437658
AGTAACA35000.044.946933
AAGAGCG7800.044.6080259
ACAAGAG36100.043.9747357
GTAGAAA36650.043.9223371
AACACGT10950.041.709486
ACGAGCT5250.041.4217349
AAGCGGA11350.040.2395442
GCACGCA8500.040.08552
GAAACAC35450.039.877394
Produced by FastQC (version 0.11.5)