FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_35_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_35_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2542155
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA32600.073.402781
TAGTAAC35100.066.506232
AACACGA20000.066.333776
AAACACG30900.063.111345
ACACGAG22200.059.7566347
CACGAGC24150.054.3312158
GTAACAA47500.048.8393634
TAACAAG48450.048.330625
ACGAGCA11100.047.674699
AGTAACA49250.047.3983543
ACGAGCC10150.046.4232259
GTAGAAA61400.044.0510861
AACACGT15050.042.3896456
GAAGCGG11700.042.1443331
CGGAGCG1557.421477E-1042.094415
ACGAGCG1401.4173565E-841.4237989
TAGAAAC66500.040.0090332
CAAGAGC57450.039.873568
CAACGCA12100.038.941799
GCACGCA6900.038.8746262
Produced by FastQC (version 0.11.5)