FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_32_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_32_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3053531
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT97200.31832000395607574No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT72750.23824876839305056No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA66280.21706018376757924No Hit
CTCTACAGCAGAGGGGCCCAGCATTTTCTTCAGCTTTCTTTCAAGCGCCA61640.20186466094498468No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCGCTTGAAAGAAAGCTGAA61130.20019446339336328No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA60100.1968213193185201No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC57950.18978029042442995No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC54420.17821990344948194No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA54100.17717193635826853No Hit
GCTCTGAGATCATCCACTTTCTCATCTAGTTCTAGTATTTCGTTGTGGAG53200.17422452891423076No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA52910.17327480873781861No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC52360.17147361529979555No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA49220.16119043821726387No Hit
GCTGTAGAGATAGGGAATGGATGCTTTGAAACCAAACACAAGTGCAACCA48700.15948749169404208No Hit
CCCATACACAATTGAATCTATCATTATTAACTTTCAAATTTTTGAGCCTG48560.1590290060916362No Hit
GTTATAATTTTACCTATTTCCCAACTTTTTGTTATTGGATCTCTCCACCA48190.15781729414242068No Hit
GAGTAGCGGTGGCAGCTGACCTTAAGAGCACTCAAGAGGCCATAAACAAG46600.15261020765795402No Hit
GCCCAAGCCATTGTTGCGAAGAATCCGTTTCCATTGGTAACGTTAGGGCA44620.146125911281071No Hit
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA44270.14497969727505633No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC43780.14337499766663578No Hit
CTTCTATTGTTAATGGATTTGTTGCTGTTTTGTTTTTGTCGTTTTTTGGG41970.13744743380695987No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG40380.1322403473224932No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA39860.13053740079927142No Hit
TTCTCATCTAGTTCTAGTATTTCGTTGTGGAGTTCATCCATGGCACCGCT39000.12772098924163533No Hit
GCTATCATCAGTGTTACAGCCAAACTGGAGGCAGCAGTTGAGTAGTAAAG37080.12143318669435484No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCA36790.12048346651794266No Hit
ATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATATTCAGTGAATCAA36640.11999223194393638No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC36420.11927175456872714No Hit
GATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTTCCAATGAAGG34900.11429391088546342No Hit
CTCTTAAGGTCAGCTGCCACCGCTACTCCATGTGCCCCATGGGATGTGTA34820.11403191911266007No Hit
AATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAAGGTACCAGCAGCT34020.11141200138462652No Hit
CTCAAAGAGTTGAGATTTTTTGTTATCTTGTTTATGGCCTCTTGAGTGCT33270.10895582851459507No Hit
GTTTATGGCCTCTTGAGTGCTCTTAAGGTCAGCTGCCACCGCTACTCCAT33020.1081371042245846No Hit
GGATTACGGGTAGCCCATACACAATTGAATCTATCATTATTAACTTTCAA32890.10771136759377914No Hit
AATATAGACCTCCTGCAAAACTATTAAAGGAAAGGGGTTTCTTCGGAGCT32500.10643415770136277No Hit
TACTAGAACTAGATGAGAAAGTGGATGATCTCAGAGCTGATACAATAAGC32410.10613941695695901No Hit
TATTGTATCAGCTCTGAGATCATCCACTTTCTCATCTAGTTCTAGTATTT32000.10479670912134183No Hit
GCCCAAAGGCCAGATGTTACCAGCAGCTGAACAGCATCTACAGAAACTAG31980.10473121117814098No Hit
ATATAGACCTCCTGCAAAACTATTAAAGGAAAGGGGTTTCTTCGGAGCTA31730.1039124868881305No Hit
GTAATAAAAGGATCCTTGCCTTTAATTGGAGAAGCAGATTGCCTCCACGA31290.10247153213771205No Hit
GGCCAGATGTTACCAGCAGCTGAACAGCATCTACAGAAACTAGCTGCAAA30660.10040834692688562No Hit
GTTCTAGTATTTCGTTGTGGAGTTCATCCATGGCACCGCTTAGTCTTTGA30610.10024460206888354No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTC1850.086.2039269
GTAACAA43350.071.581684
AACACGT10050.069.97666
AGCGTTT554.067042E-665.899698
ATTAGTA14500.063.5190281
GAGCGTT606.8146946E-660.4080477
TAACAAG52600.059.4071465
AACACGA13450.054.9825746
ACACGAG13500.054.7699667
GACTTCG400.00605156654.3761446
TAGGGTA3500.053.858285
TATTAGT11650.051.6678351
CACGAGC13950.051.4442758
ACACGTG13900.051.107827
TAGTAGT11900.050.5682953
GTAGAAA39850.050.0463561
CAACGCA6400.049.836649
GAAGCGG11550.049.60361
AAACACG29050.049.4158485
GTGCGGA450.00964021248.3343542
Produced by FastQC (version 0.11.5)