FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_31_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_31_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2258371
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT43620.19314807000267006No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC41680.1845578073753161No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA35890.15891985860604835No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT28600.1266399541970739No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT25590.11331176321339585No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC25090.1110977780001603No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA22930.10153336187898268No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC11800.0106.933741
GTAACAA58600.0104.173254
GCACGCA12250.0104.1641542
TAGTAAC66800.092.253782
CACGCAC14250.090.05343
TAACAAG69100.088.763425
CGCACTT15100.088.345135
AGTAACA75950.081.2350543
ACGCACT16900.080.651574
AAACACG25850.080.493925
GAAACAC28350.074.162894
GTAGTAA89650.069.4848861
CTCGCTC11200.066.6746753
GTCTCGC11450.065.868131
ACAAGAG93100.065.637547
GTTCGAT451.19413206E-464.444992
GAAGCGG6350.063.9531331
AACACGT10150.063.571976
ATCGCGG350.00356915462.133762145
CAAGAGC81800.061.943868
Produced by FastQC (version 0.11.5)