FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_28_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_28_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706166
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT53640.31438910399105363No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC45820.2685553457283758No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT43660.25589538180927296No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACTT33900.19869110039703056No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT33010.19347472637480762No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT29200.1711439566841679No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC27470.16100426336007165No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT22420.1314057366047618No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT21420.12554464219776973No Hit
CCCCCAGACCAAATGATGGAAGCATAACAGGCCCTTGTGAATCTGATGGG20810.12196937460950458No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT20560.12050410100775658No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC20480.1200352134551972No Hit
GCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC20280.1188629945737988No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG20230.1185699398534492No Hit
GTATTGGGATAGAGATGGTACATGATGGTGGAAAAGAGACTTGGCACTCA20090.1177493866364703No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC19920.11675300058728165No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT18770.1100127420192408No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA18450.10813719180900334No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC18420.10796135897679357No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC18420.10796135897679357No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT18300.10725802764795453No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA18230.1068477510394651No Hit
GTTTTCATAACCTCCTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA18120.10620303065469597No Hit
AGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATTGTTCAAACA17620.10327248345119995No Hit
GTAGAAACACGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT17540.10280359589864058No Hit
CTATATGTGTCAGCTTCATTATCATACTTACTATATTCGGATATATTGCT17350.1016899879613121No Hit
TCCCAATACAGGGGACATCGCATTTCTTATCTTTTATTTCGAAGCCAAAG17320.10151415512910232No Hit
CACGTGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGATAAATTTA17290.10133832229689257No Hit
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG17200.10081082380026328No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC17180.10069360191212345No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACTA151.2299407E-4145.006383
ATAATGT100.007090364145.006384
CCTGGTA100.007094715144.97664145
AGCACGC5500.0123.950881
GCACGCA5600.0122.996492
GAAACAC11050.0121.385434
CACGCAC5700.0120.838653
GTAACAA30600.0120.127854
AAACACG11600.0115.005075
GAGCAGA2150.0114.689831
TAACAAG37050.099.410585
ACGAGGA554.1654857E-1092.27684
TACTCAC259.375323E-487.029341
ACGTGCA6000.087.003842
TTTACAG1750.087.003833
TAGTAAC44550.083.000292
TAGAAAC17450.082.6827242
GAAGCGG3700.082.325061
AGAAGCG1600.081.566095
AACACGA14400.081.566096
Produced by FastQC (version 0.11.5)