FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_26_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_26_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2809867
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC48890.17399400042777824No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT46310.16481207117632257No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG45880.1632817496344133No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA40060.14256902550903655No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT39990.14231990339756292No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA38030.13534448427630205No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT36200.1288317205049207No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT35600.12669638812086126No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG34550.1229595564487572No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA33750.12011244660334458No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC33630.11968538012653268No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG33610.11961420238039737No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC30540.10868841834862646No Hit
GTATGTAAAGTATGATGGAGACCCATGGACTGACAGTGAAGCCCTTGCTC30130.1072292745528525No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA8050.086.469572
AAACACG20400.085.3039255
TAGTAAC78000.080.502842
GTAACAA77500.080.460864
AGCACGC8800.079.1154861
GTAGTAA83250.075.789311
TAACAAG83000.075.391195
ACAACGC7300.070.5067448
AGTAACA89400.070.1562653
CACGCAC10300.068.98853
AACACGA17700.068.002166
AGCGTGC4600.067.765058
ACGACGG350.00356555262.1499985
GAAACAC28050.062.039214
AACACGT9100.060.5564046
ACACGAG20150.060.0809257
GCGTGCT5700.054.6875889
CGCACTT12200.054.6784135
ACGAGCC7600.054.3696379
CAACGCA10000.054.3696339
Produced by FastQC (version 0.11.5)