FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_24_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_24_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194788
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA34130.15550476856990286No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA30860.14060583527885154No Hit
GTAGTAACAAGAGCACAAGGGGCAAAGATGACGACAGTTTTGGCTACTTC26930.12269977783731276No Hit
GTATAAAGGATAAACAAAAACTTTAAAGTACTTCTCTCTTCTGTCCTTCC24510.1116736559521922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA41700.087.8266751
TAGTAAC43750.083.85232
AAACACG27100.072.2332155
ACAACGC19250.070.797878
ACACGTG12050.065.574137
AACAACG21900.062.893037
AGTAACA58450.062.639663
AACACGA18900.062.14356
ACGAGCG2700.061.7528659
ACACGAG19000.061.4278537
GTAACAA59550.061.3608364
CAACGCA26600.060.773789
AACACGT14350.055.575496
CACGAGC20700.055.3324368
TAACAAG66900.054.9445155
ACGTGCC8450.050.6160629
ACAAGAG74200.049.3378837
CGCTATA450.00962898848.3481561
ACGAGCC11300.048.1144879
GAAACAC42050.047.7591174
Produced by FastQC (version 0.11.5)