FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_23_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_23_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2137519
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTACTTTGAGAAGGAGATCAACAATCACACTCAAGA38340.17936682668083886No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA30410.14226774124580882No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA22840.1068528513664674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC44800.089.337522
GTAGTAA46400.086.8941651
AAACACG25500.079.0455555
ACACGAG19350.067.809287
AGTAACA59750.066.984453
AACACGT11050.065.616136
GTAACAA61950.064.605664
AACACGA20300.064.290876
CACGAGC19500.062.454868
TAACAAG66150.060.28455
ACAACGC10800.055.711638
CAACGCA11850.053.8339049
ACGAGCA9600.053.614069
CAAGAGC73000.049.354318
AACAACG12350.049.306477
GTAGAAA59000.047.442671
TAGAAAC60950.046.2752462
GAAACAC44000.045.9752774
ACAAGAG87200.045.8064547
ACGAGCC6200.043.26159
Produced by FastQC (version 0.11.5)