FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_22_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_22_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077284
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTGCAAAACACTTA43780.21075596788883943No Hit
GTACTGACCAGCCATCTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG40170.1933775063977771No Hit
GGTCAGTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA36410.17527694816885897No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA33700.16223106710493126No Hit
AGTAAGATGTTTGCCTTTTCCCCTGGTTTCAGCTTTTCAAGTTCTTCAAT32850.15813918559041518No Hit
GTATTAGCAGGCTTTCTCGTTAGTGGCAAATATGACCCAGATCTTGGAGA31600.15212171277495037No Hit
ATCTGGGAGGATGCCACGTGCTTAGTAGGCTTGGAAGGACAGAAGAGAGA27930.1344544125887457No Hit
GTTCCAATGGGATGCATTTGAAGCATTTGAGAGCATAATTCCTCAGAAGA25420.12237132717529235No Hit
AAGCAGAGCTGATGATAACATATGATACACCTAAAATGTGGGAAATGGGA24820.11948294022386924No Hit
GTAGTAACAAGAGCCCTTAAAATCTACTCTTTTAGCAAAAATCCTGAATA24710.11895340261610834No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT24110.11606501566468524No Hit
ATTTATATTAGCTCAAGGCCCACCCCATGGACTCAACTGTCATTCTTTGT23980.1154391984918769No Hit
GTCCTAACTATATGCGGCAGAATGATGTCATTAAAAGGGAAAATTGAAGA23860.11486152110159228No Hit
ATATGATACACCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTGC23810.11462082218897368No Hit
CCAAAGAACTGGTGCAAAACACTTATCAATGGGTGCTAAAAAACTTGGTG23420.11274337067054867No Hit
GGTTTCAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG22850.1099994030666967No Hit
CTATTTTCCTATAATCCACAAACAGAAGTCCTAACTATATGCGGCAGAAT22530.10845893002593772No Hit
CATATGATACACCTAAAATGTGGGAAATGGGAACAACCAAAGAACTGGTG22280.10725543546284476No Hit
GATGATAACATATGATACACCTAAAATGTGGGAAATGGGAACAACCAAAG20920.10070842503961905No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC26850.089.108912
GTAGTAA32200.077.245531
AAACACG24950.073.228735
AGTAACA33450.071.5268863
GTAACAA33400.071.416894
AACACGT9850.066.981776
AACACGA17750.064.945676
ACAACGC6400.064.561448
ACGAGCT6800.063.9617969
GAAACAC29250.062.711354
ACACGAG18550.062.1343157
TAGAAAC37350.061.922942
TAACAAG40300.059.9088065
CAACGCA7250.057.9920279
CACGAGC20350.054.8573238
GTAGAAA45500.052.275431
GAAGCGG10300.052.0990331
AACAACG8150.051.5879977
ACGAGCC5500.047.448029
ACAAGAG51500.046.8721967
Produced by FastQC (version 0.11.5)