FastQCFastQC Report
Thu 6 Dec 2018
HNJM2BCX2_l02_n01_FLUB_1_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNJM2BCX2_l02_n01_FLUB_1_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1480393
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC42920.28992301368623064No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT36340.2454753568815848No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC36090.24378661612152988No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA33980.22953364410666627No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT32710.22095484104558724No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT30970.20920120535560488No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG28190.19042240810379407No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA27100.18305949838995456No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT24360.1645508996597525No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC24120.16292970853009978No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC24060.16252441074768662No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT23210.15678269216349983No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG21920.14806878984161637No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA20110.13584230673881867No Hit
AAAATGAACAATGCTACCTTCAACTATACAAACGTTAACCCTATTTCTCA20020.1352343600651989No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGCAACAAATCCAT19530.13192442817549124No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT18950.12800654961216382No Hit
GTCCTTGAGAGTGTTGGTAAATGGAACATTCCTCAAACACCCCAATGGAT18610.1257098621784891No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG18600.1256423125480869No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC18520.12510191550486932No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT18490.12489926661366271No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA18480.12483171698326054No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC17970.12138668583274848No Hit
GTATATGCTTCTCCATATTTTACTTTGAGCAATGCATTATTGTCAGGGCC17140.11578006650936609No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT17060.11523966946614853No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT16720.11294298203247383No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG16680.11267278351086502No Hit
TTATAGGAAAGCACCCAGATGTAACAGGTCTGACTTCATGGAGTATTGAA16580.11199728720684304No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT16550.11179463831563646No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA16240.10970059977316833No Hit
TGGTAGTAACATCCAATGCAGATCGAATCTGCACTGGGATAACATCGTCA16210.10949795088196175No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA15920.10753901160029802No Hit
CCCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTCCTA15720.1061880189922541No Hit
AGCATATACTGACACATACCATTCCTATGCAAACAAAATCCTAAGAACAC15640.10564762194903651No Hit
TTATCATACTTACTATATTCGGATATATTGCTAAAATTCTCACCAACAGA15470.10449927823219915No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA15430.10422907971059037No Hit
CTGTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCG15420.10416153008018818No Hit
GTATTATTATCACTATATGTGTCAGCTTCATTATCATACTTACTATATTC15380.10389133155857938No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC15030.10152709449450248No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA14840.10024365151686072No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT100.0070850058145.042421
AGCACGC1200.0114.825241
GTAACAA35000.0112.277714
GAAACAC9450.0102.042754
TAACAAG38900.0101.017665
GCACGCA1500.096.672072
AAACACG10200.094.53645
CACGCAC1600.086.0985643
AACACGA10300.080.244496
TAGTAAC50400.079.553062
CGGAGCA7000.077.6829152
ACACGAG10650.076.9109957
ACGTGCA4300.075.876332
AGTAACA53400.075.0837863
CAACGCA3700.072.486919
ACAAGAG55150.071.5011447
GTAGAAA16950.067.600891
CACGAGC12050.066.170628
CAGCGTT451.1939163E-464.445875
AACACGT2300.063.0448766
Produced by FastQC (version 0.11.5)